Dr. Stephen M. Lewis
Assistant Scientific Director, Atlantic Cancer Research Institute
Adjunct Assistant Professor, Microbiology & Immunology
Adjunct Professor in the Department of Biology at the University of New Brunswick (Saint John)
Atlantic Cancer Research Institute, Dalhousie University
Translational control, internal ribosome entry sites (IRES), biomarkers
Dr. Stephen Lewis is studying the role of translational control and novel translation initiation mechanisms in the development and progression of cancer. Translational control describes the regulation of gene expression through specific and regulated utilization of mRNA in protein synthesis. Dr. Lewis’s research is currently addressing the mechanisms underlying novel translation initiation, such as internal ribosome entry site (IRES)-mediated translation, and their role in cellular processes and disease. Specifically, he is focusing on the role of deregulated translational control in cancer and how such changes contribute to the development of the disease. These investigations may lead to the discovery of new biomarkers for specific cancers and potential therapeutic targets.
Involvement with BHCRI to date:Dr. Lewis is a BHCRI Senior Scientist and chair of the BHCRI Management Advisory Council.
Contact:Atlantic Cancer Research Institute, Hotel-Dieu Pavilion, 35 Providence Street, Moncton, New Brunswick, Canada E1C 8X3
Durie, D., S.M. Lewis, U. Liwak, M. Kisilewicz, M. Gorospe, and M. Holcik (2010) RNA?binding protein HuR mediates cytoprotection through stimulation of XIAP translation. Oncogene (in press; doi:10.1038/onc.2010.527).
Benjamin, J.J.R., P.P. Poon, S.M. Lewis, A. Auger, T.A. Wong, R.A. Singer, and G.C. Johnston (2010) The yeast ArfGAP Age1 is regulated by phospholipase D for post-Golgi vesicular transport. J. Biol. Chem. (in press; doi: 10.1074/jbc.M110.185108).
Arseneau, J.-R., M. Laflamme, S.M. Lewis, E. Maicas, and R.J. Ouellette. (2009) Multiple isoforms of PAX5 are expressed in both lymphomas and normal B-cells. Br. J. Haematol. 147, 328-338.
Zhao, T.T., T.E. Graber, L.E. Jordan, M. Cloutier, S.M. Lewis, I. Goulet, J. Cote, and M. Holcik. (2009) hnRNP A1 regulates UV-induced NF-?B signalling through destabilization of cIAP1 mRNA. Cell Death Differ. 16, 244-252.
Cammas, A., S.M. Lewis, S. Vagner, and M. Holcik. (2008) Post-transcriptional control of gene expression through subcellular relocalization of mRNA binding proteins. Biochem. Pharmacol. 76, 1395-1403.
Lewis, S.M. and M. Holcik. (2008) For IRES trans-acting factors, it is all about location. Oncogene 27, 1033-1035.
Lewis, S.M., S. Cerquozzi, T.E. Graber, N.H. Ungureanu, M. Andrews, and M. Holcik. (2008) The eIF4G homologue DAP5/p97 supports the translation of select mRNAs during endoplasmic reticulum stress. Nucleic Acids Res. 36, 168-178.
Galbán, S., Y. Kuwano, R. Pullmann, Jr., J.L. Martindale, H.H. Kim, A. Lal, K. Abdelmohsen, X. Yang, Y. Dang, J.O. Liu, S.M. Lewis, M. Holcik, and M. Gorospe. (2008) RNA-binding proteins HuR and PTB promote the translation of Hypoxia-Inducible Factor-1?. Mol. Cell. Biol. 28, 93-107.
Cammas, A., F. Pileur, S. Bonnal, S.M. Lewis, N. Leveque, M. Holcik, and S. Vagner. (2007) Cytoplasmic relocalization of heterogeneous nuclear ribonucleoprotein A1 controls translation initiation of specific mRNAs. Mol. Biol. Cell 18, 5048-5059.
Baird, S.D., S.M. Lewis, M. Turcotte, and M. Holcik. (2007) A search for structurally similar cellular internal ribosome entry sites. Nucleic Acids Res. 35, 4664-4677.
Lewis, S.M., A. Veyrier, N.H. Ungureanu, S. Bonnal, S. Vagner, and M. Holcik. (2007) Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translation. Mol. Biol. Cell 18, 1302-1311.
Graber, T.E., S.M. Lewis, and M. Holcik. (2006) An approach to whole-genome identification of IRES elements. Curr. Genomics 7, 205-215.
Ungureanu, N.H., M. Cloutier, S.M. Lewis, N. de Silva, J.D. Blais, J.C. Bell, and M. Holcik. (2006) IRES-mediated translation of Apaf-1, but not XIAP, is regulated during UV-induced cell death. J. Biol. Chem. 281, 15115-15163.
Holcik, M., T. Graber, S.M. Lewis, C.A. Lefebvre, E. LaCasse, and S. Baird. (2005) Spurious splicing within the XIAP 5’ UTR occurs in the Rluc/Fluc but not the ?gal/CAT bicistronic reporter system. RNA 11, 1605-1609.
Lewis, S.M. and M. Holcik. (2005) IRES in distress: Translational regulation of the inhibitor of apoptosis proteins XIAP and HIAP2 during cell stress. Cell Death Differ. 12, 547-553.
Lewis, S.M., P.P. Poon, R.A. Singer, G.C. Johnston, and A. Spang. (2004) The ArfGAP Glo3 is required for the generation of COPI vesicles. Mol. Biol. Cell 15, 4064-4072.