Dr. Alan Doucette, PhD
Professor, Department of Chemistry
Dalhousie University, Faculty of Science
Proteome analysis, biomarker profiling, technology development and assessment (separations and mass spectrometry)
Dr. Doucette aims to translate proteome profiling into routine clinical practice. The promise of proteomics as a tool for biomarker discovery or disease profiling has, to date, gone largely unrealized. This is due to an inherent variability in the standard approaches used to assess complex protein mixtures. His fundamental research in the Department of Chemistry challenges the standard practices involved in proteome analysis. Through his systematic efforts, Dr. Doucette is reinventing the conventional proteomics workflow, providing novel technologies to make proteomics more routine, efficient, and reliable. These strategies are applied in collaboration with others to clinically relevant samples.
Membership Status:Dr. Doucette is a BHCRI Associate Member.
Address:Chemistry Building, Dalhousie University, 6274 Coburg Road, Halifax, Nova Scotia, Canada, B3H 4J3
D. L. MacLellan, D. Mataija, A. A. Doucette, W. Huang, C. Langlois, G. Trottier, I. W. Burton, J. A. Walter & T. K. Karakach; Alterations in urinary metabolites due to unilateral ureteral obstruction in a rodent model Mol. BioSyst., 2011, online May 6, DOI: 10.1039/C1MB05080J
D. Botelho, M. J. Wall, D. B. Vieira, S. Fitzsimmons, F. Liu, & A. A. Doucette; Top-Down and Bottom-Up Proteomics of SDS-Containing Solutions Following Mass-Based Separation. J. Proteome Res. 2010, 9, 2863–2870.
J. C. Tran & A. A. Doucette; Multiplexed size separation of intact proteins in solution phase for mass spectrometry. Anal. Chem. 2009, 81, 6201 - 6209.
J. C. Tran, M. J. Wall, A. A. Doucette; Evaluation of solution isoelectric focusing as an alternative to ion exchange chromatography for charge-based proteome prefractionation. J. Chromatogr. B, 2009, 977, 803-813.
D. Mataija-Botelho, P. Murphy, D. M. Pinto, D.L. MacLellan, C. Langlois & A. A. Doucette; A qualitative proteome investigation of the sediment portion of human urine: Implications in the biomarker discovery process. Proteomics: Clinical Applications, 2009, 3, 95-105.
J. C. Tran & A. A. Doucette; Rapid & effective focusing in a carrier ampholyte solution isoelectric focusing system: A proteome prefractionation tool. J. Proteome Res, 2008, 7, 1761-1766.
J. C. Tran & A. A. Doucette; Gel-eluted liquid fraction entrapment electrophoresis: An electrophoretic method for broad molecular weight range proteome separation. Anal. Chem., 2008, 80, 1568-1573.